Pulsatile Properties of Three Dimensional iPS Cardiac Tissues Loaded Tensile Stimulation

Abstract

In this report, we measured pulsatile properties of three dimensional (3D) iPS-cardiac tissues by using micro vacuum chuck (MVC). The 3D iPS-cardiac tissue was connected to force transducer by MVC in little change of initial length and minimally stress concentration. By using MVC, we successfully applied tensile stimulation to 3D iPS cardiac tissue and measured pulsation force. It was observed that the pulsation force became larger with the increase of strain. Additionally, the pulsation force did not decrease with incubation time. This indicates that the 3D iPS cardiac tissue was not deterioration with time. The pulsation frequency of partially tissues also became large with the increase of strain and this property was increased when incubation time became over 14 days. It means that 3D iPS cardiac tissues kept maturation after 14 days.