Optical Environment Sensing

- Three-dimensional Oxygen Concentration Monitoring of Environment around cell aggregation-

Abstract

Optical environment sensors such as microbeads modified with fluorescent dyes can be applied for minimally invasive measurement of cell aggregates and three-dimensional tissues. However, compensation for the fading effect of the fluorescent dye is a big issue. We fabricated hydrogel fluorescent sensors which can compensate the fading effect by fluorescent recovery. We conducted calibration of oxygen concentration measurement. Then, we examined stability and followability to environmental oxygen concentration.