Development of cultured muscle with 3D print tendon structure

Abstract

In this article, we introduce skeletal muscle three-dimensionally cultured with extracellular matrix (ECM) and module cultured muscle with 3D print tendon structure. The 3D printed prototype was transferred by PDMS to prepare a template for muscle culture. With the tendon structure fixed to the template, myoblasts mixed with ECM were injected into the template. Cultured muscles having a tendon structure were prepared by culturing them three-dimensionally. Since the tendon structure is linked to the culture muscle in a chain form, it is possible to fix the culture muscle regardless of the cell adhesiveness of the material.