Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : May 27, 2020 - May 30, 2020
Chemotactic behavior of a monotrichous bacterium was investigated by the three dimensional measurement. An objective lens was vertically oscillated by the piezoelectric element and the position of the cell was deduced from images in focus. Three dimensional trajectories of cells swimming around an attractant were obtained with the vertical resolution of several micrometers. The turn angle (directional change) distribution, which is one of the characteristics in bacterial chemotaxis, was elucidated. The turn angles in the directional changes from forward to backward swimming are limited to large values (almost 180 degrees), whereas the angles widely range in the directional changes from backward to forward. This tendency is similar to the previous study with the two dimensional measurement.