Development of Intracellular Delivery System using Laser-Induced Shock Waves from fine patterns

Abstract

We aim to achieve a massively parallel localized intracellular delivery method for uniform transfection. The intracellular delivery of nucleic acids on a localized region is significant to control the quality of cells more precisely than the previous transfection methods. We prepared two different energy absorber materials, Ti and SU-8 / TiO₂, where TiO₂ nanoparticles were mixed with SU-8. We observed remained bubble at various laser energies and evaluated shock wave to judge whether the laser irradiation is possible to introduce molecules.