Biological Nanopore Probes Leading to Living Cell Monitoring

Abstract

This Paper describes biological nanopore probes that biological nanopores are reconstituted into the lipid bilayer formed at the tip of micro and nano electrodes. Stochastic nanopore sensing is a powerful technique for single molecular detection with high sensitivity. In addition, bio-inspired scanning ion conductance microscopy (bio-SICM) that can image topological and chemical information by using the biological nanopore probe has been reported and attract attention as a live cell imaging technique. However, it is still challenging to form stable lipid bilayers at the tip of the probe. Here, we describe formation methods of lipid bilayers using micro and nano electrodes to prepare stable lipid bilayers by supported on the electrodes. Furthermore, we demonstrate spatially resolved chemical sensing in a microfluidic channel and high throughput nanopore sensing by using a 3D printed micro chambers. From these results, we believe that our nanopore probe technology can potentially be applied as a probe of bio-SICM and a high throughput nanopore sensing platform.