Development of an Optoporation Method Applicable to Various Types of Cells Using Color Resist

Abstract

Regenerative medicine and cell therapy requirs safe and efficient transfection. Optoporation has higher viability and efficiency and it is getting attention. It is important to generalize optoporation by clarifying the dependency of each cell type. However, the dependency on cell types was not clear. We aim to realize intracellular molecular delivery independent of cell type. We conducted experiments using a simple device made of color resist and three types of cells, HeLa cells, HEK293 cells, and A-498 cells, to achieve intracellular delivery. The dependence of the cell type on cell viability and delivery efficiency was characterized.