Quantification of the acoustic radiation force applied to cells adhered to a culture dish

Abstract

Cell culture technology is a fundamental technology of biotechnology. In adherent cell culture, cell detachment from culture vessel is an essential process. Although enzyme-based cell detachment is the widely accepted technique, it damages cells. Nowadays, cell detachment technique utilizing ultrasound has been proposed. When ultrasound vibration is applied to the bottom of a culture dish, the acoustic radiation force is given to cells, resulting in cell detachment. For the implementation of cell detachment, the acoustic radiation force on cells should be quantified. Therefore, we developed the device to quantify the acoustic radiation force applied to the cells adhered to a culture dish.