To evaluate the effect of Binding Pedals (BP) on cycling exercise, we investigated the differences in leg muscle activity and mechanical efficiency between BP and Normal Pedal (NP) by measuring electromyogram (EMG) and energy expenditure during cycle exercise. Seven non-cyclists and five cyclists were performed 5-min cycling exercise under nine conditions, at three cadences (50, 75, 100rpm) and three intensities (75, 100, 150w), wearing BP or NP. BP did not increase ⊿efficiency, calculated as the ratio of increment in the external mechanical power output to the increase in metabolic energy expenditure, in short-term exercise. In the case of cyclist, BP increased vastus lateralis (VL) activity and decreased gastrocnemius lateralhead (GL) activity. In the case of non-cyclist, however, wearing BP increased GL activity and decreased Tibilis Anterior (TA) activity. Thus, there are different effects of wearing BP on the EMG activity pattern between non-cyclists and cyclists.