Generally muscle fatigue has been defined by changes of motor units as the integrated function signals of the never and the muscle as impulse signals of electromyogram. The purpose of this study was designed to evaluate with the wave signals of the recruitment pattern of the relative motor unit during maximum voluntary muscular contraction and three stepwise running speed of (40%, 60%, 80%, maximum speed record of 1500m) by the surface electromyobram (SEMG) spectra, non-invasivery. SEMG of the Rectus Femoris and the Biceps Femoris were processed for SEMG to be calculated power spectra with the time series analysis of Max Entropy Method Calculation (MemCalc) gradient at the sampling rate of 1 msec. Total Power Spectrum (area, TPS), and the decreasing gradient (β coefficient) of 1/f^β nonlinear equation in the waves of SEMG spectra were applied for SEMG spectrum to evaluated with muscle fatigue.