Abstract
Recently brain imaging techniques have relied on the close coupling between neural activities, local changes in blood flow and a change of oxygen environment. Because of the importance and widespread use of these techniques, we need to clarify their relationship. In auditory cortex, primary auditory cortex (AI) is an unique region in which acoustic frequency are topographically mapped In this study, to clarify the precise relationship between neural activity and local cerebral blood flow (CBF) in guinea pig AI, we measured CBF change and neural activity with Laser Doppler Flowmetry (LDF) and optical recording with voltage sensitive dye (RH795) during and after applying the acoustic stimulus of various frequencies (1,4,7,12 kHz). And to see how tissue pO2 respond to stimulus, we also measured tissue pO2 with an oxgen microelectrode (tip diameter<10μm).Then we visualized micro-vessels in guinea pig which effect the results of above measurements and brain imaging techniques
