Abstract
Production of artificial organs utilized in vivo and in vitro is the main topic of tissue engineering. However the method of designing their structures and the optimization of their specifications in terms of physiological functions have not been established. In this study, we designed stainless-steel flow chamber, which allows homogenous concentration of species on cell-surface, and measured oxygen consumption rate and glucose consumption rate of HepG2 cell line in different glucose concentration in steady state mass transport condition. The results provided glucose concentration dependency of these two consumption rates and glucose-oxygen consumption ratio on glucose concentration.
