Frost adhesion to a cooling surface causes many serious accidents accompanying economic losses. Therefore, it is necessary to clarify the mechanism of frost adhesion to the cooling surface both scientifically and technologically. Considering the size of frost, investigation in micro scale field is essential. However, there has been no the investigation. Thus, in this paper, a scanning probe microscope (SPM) with different radii of the probe attached to the cantilever was used, and the methods to measure frost shape such as the diameter and height and adhesion force of forest to the cooling surface and their effectiveness were investigated, varying humidity and cooling time.