Abstract
We performed the experiment in Giant Unilamellar Vesicles (GUV)-mass production that utilizes the mechanical oscillation on the silicone substrate with the rehydrated patterned-lipid-layers. The effect of the electric conductivity of rehydrating solution on GUVs' productivity was evaluated by measuring the density and the diameter distribution of GUVs that were produced in an isotonic (300 mOsmol/kg) mixture of D-mannitol (low conductivity) and KCl (or PBS) (high conductivity). It is found that GUVs are produced only by under the flow field, although the productivity of GUV becomes extremely lower as the electric conductivity becomes higher. From these results, we clarify that the electric conductivity and the flow field in the solution are important for the mass-GUY production.
