Abstract
Microscopic observations of the freezing process of plant leaf cells have been made using a directional solidification stage, in order to study the freezing mechanism of living tissue. Thus, the studies of cooling or freezing cells have been performed. However, the observation of cells around freezing point is not yet. In order to investigate the effect of cell freezing by cytoplasmic streaming, we made a temperature control stage based on directional solidification stage and observed the Egeria densa as aquatic plants. In this study, we reported that we made temperature control stage and observation of cytoplasmic streaming velocity at 5, 10 and 15℃.
