Abstract
Cryoprotective effect of dimethylsulfoxide, as the most useful cryoprotectant, and pressurized dissolution of Xenon gas was used together, and the enhancing effect of damage reduction in cryopreservation of cells was investigated. Human dermal fibroblast of monolayer culture in a culture dish was prepared for a test sample. Dimethylsulfoxide concentration was 10%. Four samples were piled and placed into a pressure-resistant chamber, Xenon gas was pressurized at approximately 0.5 MPa for dissolution, and then decompressed to atmospheric pressure. The samples were frozen from -5 to -40℃ at 0.1-1℃/min, cooled to below -150℃, and then thawed. Post-thaw cell activity was evaluated using the water-soluble tetrazolium salts assay. As a result, the effect was enhanced by pressurized Xenon gas on the conditions of a cooling rate lower or higher than 0.3℃/min, as the optimal cooling rate.
