Abstract
Active enhancement of supercooling is one of the promising techniques for high-quality cryopreservation. The object of this paper is to study the effects of alternating electric field on a supercooling of biological cell. In the experiments, onion skin cells were cooled at constant cooling rate using a directional solidification stage, with applying alternating electric field at frequency of 1kHz. As a result, it was found that the load effects of low electric current on cell enhance supercooling degree, but also those of high electric current reduce supercooling degree. The effects of electric field on surface-catalyzed nucleation on cell membrane were discussed in relation to electric current and cooling rate.
