Effect of avoiding active-transport stop on viability during cold storage of cells and protective effect by pressurized dissolution of xenon gas.

Abstract

The effect of avoiding active-transport stop on viability during cold storage of cells and protective effect by pressurized dissolution of xenon gas were investigated. Rat cardiac myocyte was selected as an experimental cell. The cultured monolayer cell in culture medium preserved at 4-8°C for 6-24 h. Cell viability was evaluated by tetrazolium salts assay. Moreover, samples were preserved at 4 and 6°C for 24 h, then the cell viability was evaluated by the pressurized xenon gas (0.5 MPa) or absence (0 MPa). Results showed that cell viability at 6-8°C in each storage time was higher than that at 4 and 5°C; and the viability was close to 100% at both 4 and 6°C in pressurized dissolution of xenon gas.