Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : October 05, 2024 - October 06, 2024
The protective effect on storage temperature and the optimal pressure by pressurized dissolution of argon gas in cold storage on a rat heart striated myocytes monolayer were investigated. The cell monolayers incubated in a culture dish for 24 h were prepared as test samples. The samples were pressurized in 0, 0.7, 0.8, 0.9, or 1.0 MPa in argon gas, and then preserved at 4 and 6°C for 24 h. The pressure-dependent cell viability was evaluated using the water-soluble tetrazolium salts assay. The results showed that protective effect in 0.8 MPa was highest for each temperature, and the protective effect at 6°C was higher than that at 4°C.
