It is expected that tissue regeneration can be promoted by using a scaffold, in which a nanofiber layer with good cell adhesion and a microfiber layer that enables three-dimensional cell migration and proliferation are laminated alternately. In this study, conditions was researched to fabricate a nanofiber layer with ϕ400-500 nm and a microfiber layer with around ϕ10 µm by electrospinning, and then the nano/micro fiber laminated nonwoven fabric was developed using a conductive spray that enables the production of thick nonwoven fabric. The fiber diameter tended to increase with the increase of polymer concentration and flow rate, and tended to decrease with the increase of the hexafluoroisopropanol fraction which has a higher dielectric constant than dioxolane. The fabricated nano/micro fiber laminated nonwoven fabric had a thickness of 629 µm. The nano/micro fiber laminated nonwoven fabric with suitable fiber distance and porosity to enhance cell migration and proliferation can be fabricated by the electrospinning method with a conductive spray.
