Abstract
The inhibitory effect of melanin-like substance on β-Amylase action was studied, and the results obtained are as follows:-
1) About 73.5% inhibition of the activity of β-amylase was caused by the preincubation of melanin-like substance at the concentration of 105γ per ml, with the enzyme solution for 10 minutes.
2) This inhibition on β-amylase is irreversible type, and the activity does not recover from inhibition by the addition of 2.58×10-2M. NaCl.
3) The inhibition ratio does not vary with pH, except in extreme alkalinity.
These results seem to suggest that the strong combination of melanin with β-amylase is occurred while the 10 minutes' preincubation of the enzyme solution with this.
