Abstract
1) The addition of 2 g of dried spinach to 100g of basal diet, consisted of milled riceand casein, interfered the calcification of the bone of rats reared with the basal diet. Theundesirable effect of spinach could be prevented by the inclusion of calcium carbonate inthe diet.
2) The treatment of raw spinach with boiling water dissolved the alkaline oxalate fromthis and, consequently, this removed the interfering action of this for calcification.
3) The boiled extract of spinach can be used as a source of vitamine B, , B, and C inthe food after removal of oxalate by“kombu”(Lioninaria japonica) or with the simultaneoussupplement of calcium carbonate.
