Abstract
For the purpose of investigating the relationship between lignin content and digestibility of herbage, the effect of various pretreatments of the colloid titration method for the determination of lignin in herbage was studied, and consequently a comparison of this method with the 72 percent H2SO4 methods was performed.
A 72 percent H2SO4 method with the pretreatment of Ellis et al yielded low results and made a clear distinction between lignin contents in accordance with the growth period of herbage.
While, the colloid titration method presented by Senju et al resulted in lower lignin values than the Ellis method, when the pretreatments with 3 percent HCl and 0.25 percent Na2CO3 was carried out.
On these experiments, it was revealed that the colloid titration method was more convenient and efficacious in dealing with much number of samples, because the method required considerably less time for an analysis.
