Abstract
The existence of rutin in the seeds of buckwheat was expected by the method of paperchromatography in our previous report, and in our recent experiments yellow crystals were isolated from the methyl alcohol extract of the seeds of buckwheat ground out of their husks.The crystals were purified by recrystallization in 30% ethyl alcohol.
They were identified with rutin by element analysis, by infra-red absorptiometry and by other methods.
The determination of rutin in buckwheat seeds and flour was done by a paperchromatographic method which consisted of the descending method using Toyo, No.50 (1cm×40cm) filter paper, 25% iso-propyl alcohol as developiong solvent, and 0.1M.aluminum chloride solution as chromogenic reagent.The rutin content was determined utilizing the correlation between the quantity of the daubed rutin and the length of the appearing spot of rutin (Rf: 0.620∼0.640).
The results were as follows;-the amount of rutin was about 9mg% in seeds, and about 6mg% in flour, and after being kept in glass bottles for six months the remaining content was, in seeds, 90.9% in the dark, and 81.2% in the light, while, in flour, 82.6% in the dark, and 70.1% in the light.
Besides, the remaining content was measured when flour was heated at 60∼100°C and when exposed to the direct sunlight.
