Abstract
1) It was revealed that four compounds derived from sugars, which could be separated by paper partition chromatography, interfered the determination of total vitamin C in sugar products by the Roe and Kuether's method.
2) Dehydroascorbic acid does not react with 2, 4-dinitrophenylhydrazine at O°C, while these interfering substances react with this in the same condition.
3) The Roe and Kuether's method was modified, based on these facts, and total vitamin C in sugar products, plant tissues, and other food products was estimated accurately by the modified method.