Eiyo To Shokuryo
Online ISSN : 1883-8863
ISSN-L : 0021-5376
Determination of Total Vitamin C in Sugar Product with 2, 4-Dinitrophenylhydrazine
Takejiroh MatsushitaTatsuroh SakaiReiko Otowa
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JOURNAL FREE ACCESS

1960 Volume 13 Issue 3 Pages 163-169

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Abstract
1) It was revealed that four compounds derived from sugars, which could be separated by paper partition chromatography, interfered the determination of total vitamin C in sugar products by the Roe and Kuether's method.
2) Dehydroascorbic acid does not react with 2, 4-dinitrophenylhydrazine at O°C, while these interfering substances react with this in the same condition.
3) The Roe and Kuether's method was modified, based on these facts, and total vitamin C in sugar products, plant tissues, and other food products was estimated accurately by the modified method.
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© Japanese Society of Nutrition and Food Science
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