Abstract
The Roe's method is well known as a 2, 4-dinitrophenyl-osazone method to detemine the amount of L-ascorbic acid in tissues.But this method is not convenient in practice due to the long hours required for production of the osazone.The authors attempted to minimize the time of reaction, and it was found that the quantitative analyses of both reduced and oxidized forms of L-ascorbic acid could be made by incubating the sample, which involved 1.2% thiourea, for 60 minutes at 50°C after adding 2, 4-dinitrophenylhydrazine.
