Abstract
High voltage paperelectrophoresis was used for the separation of the free amino acid pattern of serum, liver and urine of lysine imbalanced diet fed rat.
Electrophoresis was performed at a potential gradient of 2, 000 & 4, 000 volt/57cm and in acetic-pyridine buffer (pH3.6) for 15 and 30 minutes.
The free amino acid pattern of serum and liver of lysine imbalanced group showed no difference against that of normal rat, but the pattern of urine showed paticular difference.
On the pattern of urine of lysine imbalanced group, the increase of a-aminoadipic acid, which is an unusual amino acid in normal urine and a product from lysine metabolism, was indicated by electro-phoresis and paper chromatography.
