The following ten amino acids, arginine, cystine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophane and valine and ten vitamins, thiamine, riboflavin, vitamin B6 group, niacin, pantothenic acid, folic acid, biotin, vitamin B12, inositol and acetylcholin contained in fresh and four months storage royal jelly were determined by the microbioassay method.
