Abstract
As previously reported (in this Journal 13, 334) free α-aminoadipic acid in urine could be seperated using high voltage paperelectrophoresis.
In this paper, a simplified method of quantitative analysis was developed with paperelectrophoresis and ninhydrin reaction.The electrophoresis was performed at a potential gradient of 2kv/57cm, 15min. and 4kv/57cm, 30min.and in an acetic acidpyridine buffer (0.4: 10: 89.6) of pH 6.1. The separated α-aminoadipic acid was extracted. fromthe spot with 50% ethanol, and the amount was evaluated by ninhydrin spectro-photometry.The recovery of the amino acid by the proposed method was 98.7±4.9%.
