Abstract
Cation exchange chromatographic method proposed by S. Katz and D.G. Comb was modified for determination of 5'-ribonucleotides.
By using Dowcx-50 (H+) column, 1.0×13.0cm2, authentic 5'-ribonucleotides, AMP, GMP, IMP, ATP, and acid-soluble ribonucleotides in the muscles of both carp and scallop were separated quantitatively and determined.
The method was applied successfully to the determination of the quantities as small as 100μg of individual 5'-ribonucleotides.
