Abstract
The antibody production in the milk from the cow which had been injected with antipertussisdiphtheric vaccine 1 to 1.5 months prior to the parturition was tested during the lactation period.
1) An antigen was injected into the udders with 40ml of the vaccine (pertussis bacilli; 24 billion/ml) 3 to 7 times in 6 to 7 days intervals.
2) No significant difference in protein composition both between the immune and the control colostrum, and between the immune and the control normal milk were observed.
3) Each amount of the antibody produced in the milk was measured by the agglutination reaction with pertussis bacilli (10 billion/ml) .
In case of the immunized, the tests were positive at 2 to 3γ/ml in whey nitrogen content during the first 10 days period of the lactation; the limiting contents decreased in value during the following period and reached 25γ/ml after 160 days.
In another case of the control milk, the tests were positive at 200γ/ml of colostrum, while negative even at 3, 000γ/ml of normal milk.
4) The agglutinin was found in both the immune and the control γ-globulin fractionated with ammonium sulfate from the immune and the control milk.
The immune and the control γ-globulin gave positive results at 1.5γ/ml and at 50γ/ml of nitrogen content, respectively.
5) No antibody against diphtheria antigen was detected owing to the insufficient dose of immunifacient used.
