Abstract
In 1959, Marian Orlawski obtained glucose-6-phosphate from sucrose by the action of beer yeast and by this method the yeild was rather low, being 16-18gm. of crystalline barium salt of glucose-6-phosphate from 400gm. of sucrose.
We used starch as starting material in view of its economy and tried to get as high yeild of glucose-6-phosphate as possible by making use of potato phosphorylase and phosphoglucomutase by acetone yeast.
We examined the optimum condition for this purpose. By this method 2.4gm. crystalline barium salt of glucose-6-phosphate with a purity of 98% were obtained from 10gm. of starch.