Abstract
Optimum protein requirement being defined as the protein requirement for maintaining normal physiological functions of human body, nutritional experiments were performed with human adults who were fed various kinds of protein diet supplemented with amino acids. Total hemoglobin and serum protein amount in the circulating blood as well as urinary output of 17-KS and 17-OHCS were measured as criteria of normal physiological status of the subject. It was found that 2-3 times as much of the safe intake of essential amino acids recommended by FAO are required under the circumstances of intake of 1g/Kg/day of protein-amino acid mixture and 2500Cal/day. The pattern of essential amino acids taken may affect the function of pituitary body to produce ACTH.
