Abstract
Since the preparation of enzyme solution under mild condition is the principal step for the study of urea dehydrogenase of green algae which have rigid cell wall, the extraction of enzyme from algae was examined.
No activity was detected after extraction by sonication at 10 KC or by grinding with quartz sand or glass powder in a mortar, even though algae had been dried with acetone.
A little activity was extracted by grinding and sonication of autolysed algae with toluene. A much higher activity was obtained when fresh algae had been alternately freezed and thawed with dry ice several times before sonic extraction. However, the enzyme was inactivated by treatment with butanol.
Usual grinding of lyophilized algae in a mortar was also unfavorable. However, the active solution was prepared from algal powder which had been sufficiently ground in a ball mill for several days.
