Abstract
The binding of triphenylmethane food colors with bovine plasma albumin was studied by equilibrium dialysis method. The triphenylmethane food colors tested were guinea green B, light green SF yelowish, brilliant blue FCF, fast gree FCF and acid violet 6B. The moles of bound color per pfotein mole and free energies for the binding of colors was determined at pH 5.2-12.0. The free energies for the binding of these colors by bovine plasma albumin were from -6, 300 to -7, 500cal/mole. Although the value of the binding capacity is very difficult to determine with a high degree of accuracy, these values of the binding capacity obtained in our experiments were from 1 to 20. These values depend on the kinds and the positions of substituents on the phenyl bound with the central carbon atom in triphenylmethane colors.
