Abstract
It was devised to estimate the freshness of muscle by thin-layer chromatography, using K value which has been proposed by T. Saito et. al., in 1959.
K=B/A X 100
A: original optical density of perchloric acid extract of muscle
B: sum of optical densities of inosine and hypoxanthine eluted
Separation of inosine and hypoxanthine from nucleotides in acid extract of muscle was made on thin-layer chromatography and elution from it was in excellent recovery.
This technique is found to be more convenient than that of ion exchange chromatography. For the application of this methad, only a little amount of muscle extract (0.016-0.024ml of equal valume acid extract of wet muscle) was proved to be sufficient, and the K value estimated by thin layer chromatography was also proved to coincide with that of ion exchange chromatography.
The freshness of various kinds of muscle was successfully estimated by this method.
