Abstract
An attempt has been made to investigate growth inhibition of sake-yeast in the medium of ammonium sulfate as nitrogen source. It was found that Sacch. saké required pantothenic acid for growth and that inositol, thiamine and biotin stimulated the growth in this medium, where as inhibition of growth was caused by the addition of several amino acids including histidine, threonine and leucine.
The inhibition by L-leucine of the growth of Sacch. saké was exerted at the concentration of 10-4M in the vitamin-deficient medium, and was not reversed by the addition of sufficient amount of β-alanine and pantothenic acid. Synthesized pantoyl-L-leucine has neither activity itself, nor inhibitionon the yeast growth.
The inhibitory products from L-leucine and pantothenic acid formed in the cell-free extract are discussed.
