Abstract
In the previous papers of this series, presence of the hypocholesterolemic substance in dried mush-room and its partial purification have been reported. Further treatment by ion-exchange column chromato-graphy using Amberlite CG-4B-type 1 (CH3COO-form) resulted in isolation of two crystalline components having the structures closely related to each other. Their chemical structures were elucidated by various chemical and spectral analyses of both components and their degradation products. The results are summarized as follows.
(1) The main component C9H11O4N5 m. p. 260°C (decomp.) having hypocholesterolemic effect, was found to be identical with eritadenine (lentysine, lentinacin) recently isolated by other workers.
(2) The newly isolated minor component C9H11O3N5 m. p. 258°C (decomp.) has a structure closely related to the main one and its chemical structure was established as 2 (R) -hydroxy-4- (9-adenyl) -butyric acid (Fig. 5). This substance has only feeble hypocholesterolemic effect, if there is any. The amount of the minor component is about one fourth of the main one and obtained in a yield of 0.015% from the dried mushroom.
