Abstract
The total lipids extracted from muscles of Japanese Black Cattle were separated into phospholipid and neutral lipid fractions, and the fatty acid composition of these lipid fractions were determined by gas-liquid chromatography. Then, the triglyceride analysis was performed by means of pancreatic lipase hydrolysis.
In general, the back contained more C14: 0 and C16: 0 and less C18: 1 than the shoulder in total or neutral lipid fractions, and the round and shoulder contained more polyunsaturated fatty acids than the back in phospholipid farctions.
In beef lipids, unsaturated fatty acids were found to exist in higher concentrations in the 2-position than the 1- and 3-position of triglycerides, and on the contrary, saturated fatty acids were predominantry esterified on the 2-position of glycerols.
The triglyceride composition of beef was as follows: S3 7.2%, S2U 36.5%, SU2 42.1%, U3 14.2%. And, it was considered that the major component triglycerides in beef were POO, POP, OOO, POSt, StOO etc.
