Abstract
Composition of fatty acid and sterol constituents in the principal neutral lipid classes of rice grain, namely triglyceride, diglyceride, free fatty acid, sterolester and free sterol, was investigated. The individual lipid class was isolated from the neutral lipid fraction by preparative thin-layer silicic acid chromatography, brought to methanolysis and analyzed for the component fatty acid and sterol by gas-liquid chromatography.
The main fatty acids in triglyceride, diglyceride and free fatty acid were commonly oleic, linoleic and palmitic acid in decreasing order. The major fatty acids in sterolester fraction were linoleic, oleic and palmitic acid in decreasing order.
The chief sterols in sterolester and free sterol were β-sitosterol, campesterol and stigmasterol, among which β-sitosterol was dominating.