Abstract
Some investigators1) 2) reported that the enzymes from psychrophilic bacteria are stable without loss of the activity at 0°C or below and a low temperature is favorable for the elaboration of the enzymes.
Pseudomonas fluorescens KM (Kansai Medical School) 361=ATCC (American Type Culture Collection) 135256) (which is able to grow at 0°C) was inoculated in chemically defined rnedium and incubated at 4°C for two months and the relationship between the bacterial growth and the activity of proteolytic enzymes was observed.
Crude proteolytic enzymes, both intracellular and extracellular, would seem to have almost similar characters but some points.
Besides the auther obtained a result suggesting the presence of proteolytic enzymes bound to cellmembrane of psychrophilic bacteria.