Abstract
This paper describes the changes of lipids component and fatty acids composition in cotyledon and hypocotyl of soybean, and in endosperm and embryo of corn, during germination. The lipids were extracted from the each part with chloroform-methanol (2/1, v/v) and fractionated to non-polar lipids (NPL), glycolipids (GL) and phospholipids (PL) by silicic acid column chromatography. The fatty acids of the NPL, GL and PL fractions were analyzed by gas liquid chromatography.
During the germination, the lipid content decreased slightly in cotyledon or endosperm and increased in hypocotyl or embryo. Then, NPL in cotyledon or endosperm increased slightly and GL or PL in it decreased, while NPL in hypocotyl or embryo decreased markedly and GL or PL in it increased. The changes of the fatty acids composition in hypocotyl or embryo were markedly more than those in cotyledon or endosperm, especially, C18: 2 or C18: 3 of GL in hypocotyl or embryo increased markedly.
