Abstract
Rapid and accurate methods for measuring calcium in laying hen plasma and shell gland fluid by atomic absorption spectroscopy have been examined in the present work.
The established procedure was briefly given as follow : Into a 25ml volumetric flask were pipetted 0.5ml of plasma or 1ml of shell gland fluid, and 2.5ml of strontium chloride solution (40, 000 ppm Sr). The mixture was made up to 25ml with distilled water. Using the dilute fluid prepared as above, the calcium content of plasma or shell gland fluid was determined with a nitrogen oxide-acetylene flame by atomic absorption spectroscopy.
Thus, calcium in laying hen plasma and shell gland fluid could be determined on directly dilute samples without prior removal of any constituents. Recoveries of calcium added were satisfactory; values recovered were 100-107% for plasma and 97-100% for shell gland fluid.
