Abstract
The action of Na-cholate on Staphylococcus epidermidis has been studied through the changes of salt tolerance of the organisms. Though the organisms were able to grow in the 12% NaCl or 0.1% Na-cholate medium, no appreciable growth was observed in the 4% NaCl medium containing 0.2% Na-cholate under the same conditions. When a large quantity of NaCl was added to the standard medium containing 0.2% Na-cholate at the middle of logarithmic phase, a rapid destruction of test organisms was observed. Furthermore, exposure of the organisms to the mixture of NaCl and Na-cholate led to a progressive increase in the release of the substances which show absorbance at 260nm.
