Abstract
This experiment was conducted to determine the nutritive value of whole leaf proteins of Japanese radish. At first, three kinds of protein samples; freeze-dried leaf (FL), pretreated leaf (PTL) obtained by our method, and hot 0.3% alkali-containing 60% ethanol soluble proteins (WLP) were prepared.
The true digestibility and biological values of the protein samples were determined by the nitrogenbalance method with adult rats. The former were obtained as follows: FL 79.7%, PTL 79.5%, and WLP 57.2%, and the latter as follows: FL 62, PTL 81, and WLP 42.
The coefficient of in vitro digestibility was determined by the method of pepsin-trypsin digestion. The coefficient of digestibility of WLP by pepsin digestion was significantly lower than that of FL, but it was raised to that of FL by pepsin-trypsin digestion.
From these results it was concluded that hot 0.3% alkali-containing 60% ethanol soluble protein (WLP) was not suitable for the protein-source of foods and fodders.
