Abstract
Effects of CASF (Ca2+-activated sarcoplasmic factor) treatment on myofibrils prepared from rabbit skeletal muscles during storage were investigated. In this report, myofibrils prepared from at death and 7 days stored muscles were named 0-FM and 7-MF, respectively.
The amount of products released from 7-MF by CASF was less than that from 0-MF, and this observation indicates that the amount of substrate available for CASF in 7-MF is less than that in 0-MF. Acrylamide gel electrophoresis showed that the amount of α-actinin, one of main constituents of Z-line, released from 7-MF was less than that from 0-MF. CASF treatment on 0-MF for 30min caused the increase of Mg-ATPase activity, and CASF treatment on 7-MF for same period caused the decrease of Mg-ATPase activity.
The obtained results in this report suggest an important role of CASF on the changes in myofibrils caused by postmortem storage of the muscle.
