Eiyo To Shokuryo
Online ISSN : 1883-8863
ISSN-L : 0021-5376
Extraction of Proteins of Starch-rich Foods for Amino Acid Analysis
Gen-ichi DANNOKazuki KANAZAWAMasato NATAKE
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1978 Volume 31 Issue 5 Pages 481-484

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Abstract
A method for the quantitative extraction of proteins in cereals and beans is described. By extraction of flour with sodium dodecyl sulfate in combination with 2-mercaptoethanol, 96 to 99% of the total flour nitrogen solubilized into clear supernatant. No effect of sodium dodecyl sulfate and 2-mercaptoethanol on the recovery of amino acids during hydrolysis with 6N hydrochloric acid was observed.
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© Japanese Society of Nutrition and Food Science
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