Abstract
The effect of fructose feeding on fructose transport was studied in rat intestinal everted sacs.
Small intestinal everted sacs were made from rats (120-150g) meal-fed a high fructose diet for two weeks.
The time course of the fructose uptake into the everted sacs was observed.
The fructose uptake of rats meal-fed the high fructose diet was approximately 15% greater than that of rats meal-fed the glucose diet. With addition of NaF to the incubation medium, the fructose uptake of rats meal-fed the high fructose diet decreased to approximatelly two-thirds the original amount, but the fructose uptake of rats meal-fed the high glucose diet did not change much from that without NaF in the incubation medium.
Na+, K+-ATPase activity of the everted sacs showed a remarkable decrease in the presence of NaF.
