Abstract
In regard to inhibition with polyphenols against formation of the strong carcinogenic nitrosamines, the reaction of nitrite with polyphenols, especially catechol, was studied in relation to their browning reaction.
1. Polyphenols reacted comparatively rapidly with sodium nitrite, causing remarkable browning. The rate of browning decreased in the order of gallic acid>tannic acid>pyrogallol>catechol>p-benzo-quinine.
2. The reaction of sodium nitrite with catechol was accellerated at a strong acidic pH range (pH 2.0 and pH 4.0) and caused an intense browning color followed the rapid decrease of sodium nitrite.
3. The browning reaction products of sodium nitrite-catechol system was fractionated by Sephadex G-25 column chromatography into three components. The formation of the reducing browning reaction products showing absorption UV range and the reducing ability to the 2.6 dichlorphenolindophenol was reconfirmed from the results of paper chromatography.
4. Catechol inhibited the formation of nitros dimethylamine in the reaction of dimethylamine and sodium nitrite.
