Abstract
The purpose of this study was to investigate the effect of three kinds of storage solutions on the tooth replantation in Japanese monkeys(Macaca fuscata). Examined solutions were 1) organ preservation solution (UW), 2) milk (ML), 3) new solution for tooth preservation (TP), and 4) isotonic sodium chloride solution (DS) as control. Maxillary lateral incisors of 18 animals were extracted and immersed in the storage solutions for 24 hours at 4℃. Root canal treatment was performed during replantation using finger pressure to reposition the tooth.
The replanted teeth and their periodontal tissues were examined histopathologically 2, 4, and 8 weeks after replantation. All replanted teeth retained normal mobility except DS. Histopathologically, the rearrangement of the periodontal ligament around the replanted teeth could be observed at 8 weeks of storage in UW, ML and TP, but root resorption and dent-alveolar ankylosis were observed after storage in DS. Tissues containing fibloblasts were found in a narrow zone in the middle of the periodontal ligament 2 weeks after replantation. The periodontal ligament around the teeth stored in three kinds of solutions revealed no inflammation and that fiber bundles were arranged in a pattern of functional orientation. However, cellular components and fiber arrangement were more numerous and definite in the periodontal ligament stored in UW than those in ML and TP after 4 and 8 weeks. Slight resorption and new formation of cementum were detected on the surface on which numerous cementoblasts existed. These results suggested that examined storage solutions are useful for teeth preservation.
